1.19.2 Opening the Primer Design Dialogs

  • You can access the Primer Design dialog in three ways:
    • By first making your sequence selection, view the length and properties of your selection on the bottom bar and then right clicking on the project sequence and selecting "Create primer from selection or "Design primers with Primer3" from the drop down menu (Figure 1.19.2.1)
    • By clicking on the "Primers" icon in the editing toolbar (Figure 1.19.2.2)
    • Or by clicking on the Tools menu in the menu bar and then selecting "Primers." (Figure 1.19.2.3).
  • In all cases you can choose to create a primer from your selection (for manual design) or to auto design primers with Primer3.


    Figure 1.19.2.1: Access Primer Design dialog by right clicking on selected portion of sequence.

    </div>


    Figure 1.19.2.2: Primer design icon in the toolbar.

    </div>


    Figure 1.19.2.3: Accessing Primer Design from the Tools menu in the menu bar.

    </div>