2.4 Manual annotation of a sequence
Manual annotations can be created as either main annotations or sub annotations.
Creating main annotations
To annotate a sequence, go to the DNA view, select it, right click and choose "Annotate selection" from the drop down menu (Figure 2.4.1).
A new tab of "More properties" will appear where all the annotation details can be inserted and modified (see section 1.2.10) (Figure 2.4.2). For instance; a name may be given as "My new seq'", the feature key can be picked to be "reporter", etc.
The new main annotation will be displayed as all other main annotations (Figure 2.4.3).
Creating sub annotations
Sub annotations can be created via selecting a sequence, right clicking and choosing "Add sub annotation" (Figure 2.4.4).
The new annotation will be displayed below the main DNA layer as well as all sequence views. Clicking on one annotation in one of the views will highlight the corresponding sequence in the adjacent view. Hovering over the annotation will show the annotation details. Right clicking will allow to edit the annotation details/ Delete the annotation/ Set the sub annotation as main/etc. (Figure 2.4.5).
The new annotation will also appear in the annotation table with the default properties: Name- "New annotation"/ Feature key- "Unknown"/ Type- "Manual"/ Strandness- "Plus"/ Identity- 100% (Figure 2.4.6). For annotation editing, see section 1.26.
Figure 2.4.6: New manual sub annotation as displayed in the Annotation table.