BLAST sequences At NCBI
For blusting a sequence through NCBI in SnapGene you should first select the appropriate DNA sequence and then go to “Tools”-”BLAST selected DNA” from the main menu (Figure 3.4.17.1). This will open the NCBI “Standard Nucleotide BLAST” link. The same can be done for primers as well as CDS for protein BLAST.
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In Genome Compiler you should select your sequence and either right click (Figure 3.4.17.2) or go to “Tools”-”Blast” (Figure 3.4.17.3) to select the appropriate NCBI blast program. This will lead you to the online NCBI search whereby your query sequence will appear and you’ll be able to change the search parameters.
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