1.19.6 Auto Design Primers Dialog
The Auto design primers (Primer3) dialog (Figure 1.19.6.1) is divided into three sections. Each section is detailed below.
Figure 1.19.6.1: The Auto design primers (Primer3) dialog.</div>
You can add a description and change the name of the primer. To edit the selection, change the start and end locations, or select a different sequence and click "Use selection" (Figure 1.19.6.2).
Figure 1.19.6.2: The Auto design primers (Primer3) dialog: Primers name, description, selection.</div>
- Select a primer task from the drop down menu.
In the expanded "General" section you will find primer parameters. The default values for all primer parameters can be edited (Figure 1.19.6.3).
Figure 1.19.6.3: The Auto design primers (Primer3) dialog: general primer parameters.</div>
More parameters can be found under "Advanced" (Figure 1.19.6.4) which can also be edited if desired.
Figure 1.19.6.4: The Auto design primers (Primer3) dialog: advanced.</div>
- Click "Generate" to generate the primers.