Thermodynamics

  • In order to view the thermodynamics parameters of a chosen DNA/RNA part in SnapGene you should select the requested part in the sequence tab to view the Tm (Figure 3.4.12.1). The GC will be shown at the top left of the screen (Figure 3.4.12.1). Selecting “Tools” – “Show DNA Molecular Weight” from the main menu (Figure 3.4.12.2) will display more parameters(Figure 3.4.12.3).


    Figure 3.4.12.1: Tm & GC content as displayed in SnapGene.

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    Figure 3.4.12.2: Launching additional DNA molecule properties in SnapGene.

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    Figure 3.4.12.3: Additional DNA molecule properties in SnapGene.

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  • In Genome Compiler when you choose to view a single project at the top right (Figure 3.4.12.4), for each part you select in the project the appropriate DNA/RNA thermodynamics as well as protein parameters will be automatically displayed on the bottom of the screen (Figure 3.4.12.5).


    Figure 3.4.12.4: “Single project view” in Genome Compiler.

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    Figure 3.4.12.5: DNA/RNA thermodynamics as well as protein parameters display is Genome Compiler.

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