2.2 Gene Amplification

This simple flow describes the process of gene amplification.

Step 1: Finding your Gene of Interest

• Find your gene of interest. This can be done:
  • via our NCBI search tool (see section 1.9)
  • by finding information about the gene location in an article and using our ”Go to” feature (see section 1.6)
  • by using our project search tool (see section 1.8)
  • or by importing a file (see section 1.4)

• In this example, the hybD gene is found via the project search tool (Figure 2.2.1).

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  Figure 2.2.1: Searching for the hybD gene using the search box.

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Step 2: Commenting on the Part

• Add a comment to the part (Figure 2.2.2) by right clicking on the part and selecting ”Add comment” in the drop down menu (see section 1.11).

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  Figure 2.2.2: Comment on the hybD gene.

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Step 3: Designing Primers

• Design primers (see section 1.19). You can auto design primers (Figure 2.2.3).

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  Figure 2.2.3: Auto design primers for the yjhQ gene.

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Step 4: Ordering

• Place your order (see section 1.13).