Transferring single/multiple Oligos from SnapGene to Genome compiler

Details about this feature can be found in the main Genome Compiler user guide:

-See section 1.3 for Importing/Exporting files.

-See section 1.18.5 for Primer Summary Table information.

-See section 1.18 for Primer Design.

  • Export from SnapGene as csv or txt file..

    In SnapGene first select the required primer and then open the “Primers” drop down menu from the main menu, choose “Export Selected Primer...” (Figure and then “Comma-separated values” or “Tab- delimited values” for the file format (Figure Both formats creates a file with two columns, the first one is the primer’s name and the second is it’s sequence. These files can be directly imported to Genome Compiler as explained below.

    Figure Export primers using the main menu in SnapGene.


    Figure Choose “Comma-separated values” or “Tab- delimited values” when exporting primers from SnapGene.


  • If you already have a csv/txt primers file you can import many primers at once, just be sure to rearrange the list in the following columns order (Figure




    Figure Primers table example in Excel.


  • Import primers table to Genome Compiler.

    Starting with your target project open, navigate to the menu bar and select “File,” then “Import,” and then “Import Primers” (Figure The “Import Primer” dialog box will then open where you can upload your primer library (Figure Select “choose a file” to choose the appropriate file. The primers will then be imported into the Import Primers dialog where you can select the checkboxes of the primers you would like to detect in the project and then “Import”. The primers which are complementary to your sequence will appear on the sequence (Figure as well as in the Primer Summary Table for that specific project (Figure (see section 1.18.5. for Primer Summary Table information).

    Figure Opening "Import Primers" dialog box in Genome Compiler.


    Figure Upload your primer library through the "Import Primers" dialog box.


    Figure Complementary primers will appear in the sequence view.


    Figure Complementary primers will appear in the project Primer Summary Table.


    Note: If the primers you imported are not complementary to anywhere on the sequence then they will not be added to your project or saved anywhere inside the program. For more information on importing/exporting files please refer to section 1.3.