1.22.7 Step 3 - Gibson Assembly: Select Fragments
The next step is the addition of fragments and spacers for your Gibson Assembly.
How to add Fragments and Spacers
Add fragments and spacers by clicking the + icon at the top of the left panel (Figure 22.214.171.124).
- This will open a new tab whereby you can upload your fragment sequence (Figure 126.96.36.199) or enter a short spacer sequence and the name. (Figure 188.8.131.52).
You can add more fragments or spacers anywhere in the assembly by clicking on the + icon anywhere in the backbone at any time.
How to Import Fragments
If you choose to add a fragment, you can upload it the same way as uploading the backbone in the previous step. You can either drag and drop the project containing the fragment, or expand the project in the Materials box which contains the fragment (Figure 184.108.40.206) and then drag and drop that fragment, or choose a file from your computer, or type/paste sequence (Figure 220.127.116.11).
- Set the fragment by typing coordinates into the "Start" and "End" fields or selecting sequence in the right panel and then either "Select all" or "Use Selection."
The coordinates of your selection will automatically be added into the fields (Figure 18.104.22.168).
- You can switch views in the right panel to zoom into the sequence and adjust the caret to fine tune your selection.
- Note: Fragments smaller than 120bps are not permitted in the Gibson Assembly.