3.3.15 New Feature a.k.a Manual annotations
Details about this feature can be found in the main Genome Compiler user guide:
See section 2.4 for Manual annotations.
See section 1.25 for annotations editing.
See section 1.2.10 for Properties Section.
In ApE in order to define a sequence as a new feature you should select the appropriate sequence and choose “Features”-”New Feature” from the main menu (Figure 220.127.116.11). This will open the “Edit feature” dialog whereby you can define the new feature.
- In Genome Compiler Manual annotations can be created as either main annotations or sub annotations.
Creating main annotations
To annotate a sequence, go to the DNA view, select it, right click and choose ”Annotate selection” from the drop down menu (Figure 18.104.22.168).
A new tab of "More properties" will appear where all the annotation details can be inserted and modified (see section 1.2.10) (Figure 22.214.171.124). For instance; a name may be given as "My new seq'", the feature key can be picked to be "reporter", etc.
The new main annotation will be displayed as all other main annotations (Figure 126.96.36.199).
Creating sub annotations
Sub annotations can be created via selecting a sequence, right clicking and choosing "Add sub annotation" (Figure 188.8.131.52).
The new annotation will be displayed below the main DNA layer as well as all sequence views. Clicking on one annotation in one of the views will highlight the corresponding sequence in the adjacent view. Hovering over the annotation will show the annotation details. Right clicking will allow to edit the annotation details/ Delete the annotation/ Set the sub annotation as main/etc. (Figure 184.108.40.206).
The new annotation will also appear in the annotation table with the default properties: Name- "New annotation"/ Feature key- "Unknown"/ Type- "Manual"/ Strandness- "Plus"/ Identity- 100% (Figure 220.127.116.11). For annotation editing, see section 1.25.