1.21.1 Opening the Cloning Wizard for Restriction Ligation

You can open the "Cloning Wizard" in one of the following ways:

• Click the "New Construction icon" in the toolbar or from the "File" menu select "New Construction Project" (Figure 1.21.1.1).

  ---

  

  Figure 1.21.1.1: The “Cloning Wizard” icon in the toolbar and File Menu.

  </div>

  ---

• In the linear or circular view select the insert sequence you would like to clone, right click and select "Clone to..." (Figure 1.21.1.2) or from the "Edit" menu select "Clone selected part."

  ---

  

  Figure 1.21.1.2: "Clone to..." in the right click drop down menu.

  </div>

  ---

• Open the project containing the vector you would like to use, in the linear or circular view right click and select "Insert fragment..." from the drop down menu (Figure 1.21.1.3) The last two options will automatically add the insert sequence or the destination vector in the cloning wizard flow.

  ---

  

  Figure 1.21.1.3: "Insert fragment/s..." in the right click drop down menu.

  </div>

  ---